English
Controlled loading of multiple active species on metal-organic frameworks (MOFs) and maintaining monodispersity has long been a huge challenge because MOFs are prone to agglomeration and collapse in water. Here, we develop an emerging self-stable dual-enzyme delivery system with long-lasting monodispersity that enables synergistic cancer starvation and photothermal therapy (PTT) without any chemotherapy. The loading of glucose oxidase (GOx) confers a strong coordination interaction with the MOF, which not only confers strong self-stabilizing monodispersity without any surface modification, but also guarantees catalytic and photothermal conversion properties Uniform deposition of Au nanoparticles and GOx-like nanozymes. Our design establishes an effective synergistic therapeutic platform to generate reactive oxygen species, reduce mitochondrial membrane potential, and induce apoptosis. Immunofluorescence studies confirmed that Caspase 3 protein activated the apoptosis pathway and significantly inhibited the proliferation of cancer cells. Our work provides new insights into stabilizing MOFs by GOx without any chemical modification and constructs an unprecedented dual-enzyme delivery system with excellent cancer synergy at very low doses without using any chemotherapeutic drugs. treatment effect.
