English
The biosafety issues associated with fecal microbiota transplantation (FMT) limit its clinical application in the treatment of ulcerative colitis (UC). The gut microbiota secretes abundant extracellular vesicles (Gm EVs), which play a crucial role in communication between bacteria and between bacteria and hosts. Here, curcumin nanocrystals coated with tea lipid/Pluranic F127 are used（ CN@Lp127s ）To train gut microbiota, these nanocrystals can maintain stability during passage through the gastrointestinal tract. Compared with FMT, Gm EVs from healthy mice significantly improved the therapeutic effect on UC by reducing colonic inflammation, restoring colonic barrier function, and rebalancing gut microbiota. Surprisingly, compared to receiving FMT from healthy mice, Gm EV from healthy mice, and CN@Lp127 Compared to the FMT group of healthy mice trained CN@Lp127 The Gm EV obtained from trained healthy mice showed better therapeutic effects on UC. Oral administration CN@Lp127 The Gm EV of trained healthy mice can not only alleviate colitis, promote mucosal repair, and regulate gut microbiota, but also regulate purine metabolism to reduce uric acid levels, thereby significantly improving UC. This study demonstrates the therapeutic effect of Gm EV extracted from the gut microbiota trained with nanomedicine in regulating the immune microenvironment, microbiota, and purine metabolism of the colon for UC treatment. These EVs provide an alternative platform to replace FMT as a treatment for UC.
